Inhibition of granulocyte erythrophagocytosis by HLA antisera

Inhibition of granulocyte erythrophagocytosis by HLA antisera.

The ability of HLA antisera to inhibit granulocyte erythrophagocytosis (EP) of opsonized red blood cells (RBC) was evaluated. Human granulocytes (PMN) were separated from heparinized whole blood by the Ficoll-Isopaque technique and suspended in McCoy's medium. EP occurred when the PMN were incubated with opsonized RBC. For six HLA antibody specificities evaluated, prior incubation of PMN with H...

Inhibition of Eimeria acervulina Sporozoite Invasion by Rabbit and Chicken Antisera Using ISI Assay

Inhibition of the Fc receptor of human lymphoid cells by antisera- recognizing determinants of the hla system

The inhibitory effect of HLA antisera on Fc receptors of human lymphoid cells was investigated. The ability of lymphoid cells to form rosettes (FcRFC) with antibody-coated sheep red blood cells and to function as effector cells (K cells) in antibody-dependent cell-mediated cytotoxicity were used as assay systems. We found that antisera recognizing determinants of the HLA-A, -B, and -C series ha...

Advantages of Placenta as a Source for Mass Production of HLA-Antisera

Human Leukocyte Antigens (HLA) of fetus are responsible for induction of HLA antibodies in mother. Exposure to the mismatched paternal HLA antigens in the fetus gives rise to a polyclonal antibody response against paternal HLA antigens. HLA antibodies are present in the maternal circulation of the placenta and are absorbed onto the placenta [1]. The major source of HLA antisera is from sera of ...

Precipitation and inhibition of carboxypeptidase by specific antisera.

Quantitative immunochemical techniques have been employed in the study of enzyme systems as a method of assessing the homogeneity of the enzyme protein. In addition, when the antibody acts as an enzyme inhibitor, useful information can frequently be obtained. Such studies have recently been reviewed by Marrack (1). With crystalline carboxypeptidase as an antigen, specific antibodies were obtain...